Journal: iScience

Article Title: Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques

doi: 10.1016/j.isci.2025.112232

Figure Lengend Snippet: DNA/LION vaccine induces durable T cell responses in rhesus macaques (A and B) Spike-specific T cell responses were measured in PBMCs over time by flow cytometry in (A) study 1, as IFN-γ + T cells, and (B) study 2, as cytokine + (IFN-γ, TNF-α single- and/or double-positive) T cells. Scatterplots with median line and minimum and maximum values are shown. The pie chart depicts the frequency of spike-specific IFN-γ, TNF-α single- and/or double-positive T cells. (C) Comparison of CD4 + and CD8 + IFN-γ + spike-specific T cell responses induced by DNA/LION (study 1, group 1) and by DNA(IM) prime followed by DNA/LION boost (study 1, group 2) and a historical study examining spike DNA vaccination by EP alone ( n = 4) or co-immunized with adjuvanted spike protein ( n = 4). (D) Frequency of spike-specific cytokine + T cells CD4 + and CD8 + T cells at the day of V4 and 2 and 3 weeks later (study 1). Bars indicate mean values. p values from generalized estimating equation (GEE) and are defined as ≤0.05, ∗; ≤0.01, ∗∗; ≤0.001, ∗∗∗; ≤0.0001,∗∗∗∗. (E) Frequency of cytokine + spike-specific CD8 + T CM (CD28 + CD95 + ) and T EM (CD28 − CD95 + CCR7 − ) after V4. Scatterplots with mean values are shown. (F) Proportion of cytokine + CD8 + T cells expressing EOMES and/or T-bet (mean of 8 animals). (G) Manually gated spike-specific cytokine + CD107a + CD8 + T CM and T EM cells (V4 [blue], V4w2 [red], V4w3 [green]) were overlaid in histograms depicting expression of EOMES, T-bet, GzmB, and Ki67 (mean fluorescent intensity [MFI] as mean of 8 animals at each time point). Gray dotted line marks the border between the negative and positive population.

Article Snippet: Mouse anti-human CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience , Thermo Fisher Scientific , Cat#12-1079-42; RRID: AB_10853326.

Techniques: Flow Cytometry, Comparison, Expressing

Journal: iScience

Article Title: Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques

doi: 10.1016/j.isci.2025.112232

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience , Thermo Fisher Scientific , Cat#12-1079-42; RRID: AB_10853326.

Techniques: Staining, Clinical Proteomics, Recombinant, Binding Assay, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

Journal: Cell Reports Medicine

Article Title: A bispecific T cell engager recruits both type 1 NKT and Vγ9Vδ2-T cells for the treatment of CD1d-expressing hematological malignancies

doi: 10.1016/j.xcrm.2023.100961

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD107a-PE (clone H4A3) , Miltenyi Biotec , Cat# 130-095-515; RRID: AB_10828805.

Techniques: Blocking Assay, Recombinant, Variant Assay, Control, Binding Assay, Red Blood Cell Lysis, Isolation, Cell Isolation, Expressing, Software, FCAP Assay